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Reconstitution Protocol

CJC-1295 (No DAC) + Ipamorelin Reconstitution Protocol: Bacteriostatic Water & Aliquoting

3 min read•Published 2025-04-04•Quality Peptide USA Research

This co-lyophilized research blend pairs CJC-1295 without the Drug Affinity Complex (a 30-amino-acid GHRH analog) with Ipamorelin, a highly selective pentapeptide ghrelin-receptor agonist. The combination is used in growth hormone secretagogue research to study simultaneous activation of GHRH and GHS-R1a receptor pathways in pulsatile GH release models.. This guide covers reconstitution protocol for CJC-1295 (No DAC) + Ipamorelin — written for laboratory researchers sourcing US-synthesized peptides from Quality Peptide USA.

Materials Required

To reconstitute CJC-1295 (No DAC) + Ipamorelin, prepare: a sealed vial of CJC-1295 (No DAC) + Ipamorelin, bacteriostatic water (0.9% benzyl alcohol), a sterile insulin or tuberculin syringe with appropriate needle gauge, an alcohol prep pad, and a clean laminar flow surface or comparable workspace. Quality Peptide USA stocks both bacteriostatic water and individually wrapped alcohol prep pads as companion lab supplies.

Standard Reconstitution Steps

1. Allow the lyophilized CJC-1295 (No DAC) + Ipamorelin vial to equilibrate to room temperature for 5–10 minutes. 2. Wipe both vial stoppers with an alcohol pad. 3. Draw the desired volume of bacteriostatic water (commonly 1–3 mL per vial depending on study concentration). 4. Slowly inject the water down the inside wall of the peptide vial — do not inject directly onto the powder. 5. Swirl gently to fully dissolve. Do not vortex or shake aggressively. 6. Inspect the solution: it should be clear, particulate-free, and free of color.

Concentration Math

With 5mg + 5mg per vial, common research dilutions include 1 mL bacteriostatic water (yielding 5mg + 5mg/mL), 2 mL (5mg + 5mg/2 mL), or 3 mL for finer titration. Match the dilution to the volumetric precision of the syringe used for downstream aliquoting. Use the molecular weight (available on the certificate of analysis) on the certificate of analysis to convert to molar concentration when required.

Aliquoting and Single-Use Storage

For longer-term studies, aliquot reconstituted CJC-1295 (No DAC) + Ipamorelin into low-protein-binding microcentrifuge tubes immediately after reconstitution, then store at −20°C in a frost-free freezer. Avoid repeat freeze/thaw cycles — each cycle increases the risk of aggregation and degradation. Working aliquots refrigerated at 2–8°C should be used within the window listed on the CJC-1295 (No DAC) + Ipamorelin product page.

Research Use Only

All content is for laboratory and in-vitro research contexts only. Not intended to diagnose, treat, or prevent any condition. Not for human or veterinary consumption.

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